Optimal processing of bone marrow trephine biopsy: the Hammersmith Protocol

Optimal processing of bone marrow trephine biopsy: the Hammersmith Protocol.

Specimens of bone marrow trephine biopsy (BMT) are transported and fixed in acetic acid-zinc-formalin fixative, decalcified in 10% formic acid-5% formaldehyde and processed with other specimens to paraffin-wax embedding. Sections, 1-microm-thick, are cut by experienced histotechnologists and used for haematoxylin and eosin, Giemsa, reticulin silver and other histological stains. Further, all im...

Leaders Bone marrow trephine biopsy

Trephine biopsies of the bone marrow should be carried out, when clinically indicated, by trained individuals following a standard operating procedure. A bone marrow aspiration should be performed as part of the same procedure. For patient safety and convenience, biopsies are usually performed on the posterior iliac crest. The biopsy specimen should measure at least 1.6 cm and, if it does not, ...

Bone marrow trephine biopsy in lymphoproliferative disease.

Introduction Examination of bone marrow biopsy specimens is a valuable diagnostic procedure in patients with known or suspected lymphoproliferative disorders. ' A bone marrow biopsy specimen may be diagnostic in patients without lymphadenopathy or when there is no conclusive histological evidence from other sites. In some conditions, such as hairy cell leukaemia,2 it is the primary diagnostic p...

Bone-marrow aspiration and trephine biopsy.

The Role of Bone Marrow Aspiration and Bone Marrow Biopsy in Diagnosis of Bone Marrow Metastases

Background: Bone marrow is the site of many malignant disorders and it is one of the common places for solid tumors to metastasize. Examination of the bone marrow aspirate and biopsy is a routine procedure performed for assessment of various conditions such as cytopenias, hematologic neoplasms, nonmalignant disorders and metastatic neoplasms. Methods: The patients were referred to the Hema...